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amino acid 1 249  (Addgene inc)


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    Addgene inc amino acid 1 249
    Amino Acid 1 249, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amino+acid+1+249/pSpCas9n(BB)-2A-GFP+(PX461)+(Plasmid+%2348140)/pm38012399-526-15-19
    Average 96 stars, based on 270 article reviews
    amino acid 1 249 - by Bioz Stars, 2026-10
    96/100 stars

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    Plasmid Preparation:

    Article Title: Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice.
    Article Snippet: .. Additionally, the pCBH-SpG-1249-APOBEC3AY130F-T2A-EGFP plasmid was engineered by inserting APOBEC3A into SpG on the position of amino acid 1,249 (PX461, Addgene plasmid no. 48140)49. ..



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    amino acid 1 249  (Addgene inc)
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    Amino Acid 1 249, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amino+acid+1+249/pSpCas9n(BB)-2A-GFP+(PX461)+(Plasmid+%2348140)/pm38012399-526-15-19
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    gsdm nt (c3vn8, amino acids 1–249)  (GenScript corporation)
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    Full-length or N-terminal pejvakin expression does not induce pyroptosis. A Structural alignment of the molecular models of mouse GSDMA3 NT (6CB8) (brown) with the model for the template generated <t>human</t> <t>PJVK</t> NT (cyan) (left panel). Structural alignment of human GSDME NT (brown) with human PJVK NT (Cyan) both generated from the mouse GSDMA3 NT template-based model (middle panel). Structural alignment of mouse GSDMA3 NT (6CB8) (brown) with the template-based model generated for jawless Chordate lancelet fish ( Branchiostoma floridae ) <t>GSDM</t> NT (Cyan) (right panel). B Measurement of LDH release, IL-1β release, and Yo-Pro-1 + uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express human full length PJVK, PJVK NT , full length GSDMD, or GSDMD NT . C Measurement of LDH release, IL-1β release, and Yo-Pro-1 + uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express lancelet GSDM NT (blue), shark PJVK NT , or GSDME NT (dark yellow) or mouse GSDMD NT (red). In B and C, each point represents an individual assay with at least n =3 to 6 different assays. * p <0.05, ** p <0.01, and *** p <0.001; Kruskal-Wallis test with Dunn’s multiple comparison post-test
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    Full-length or N-terminal pejvakin expression does not induce pyroptosis. A Structural alignment of the molecular models of mouse GSDMA3 NT (6CB8) (brown) with the model for the template generated <t>human</t> <t>PJVK</t> NT (cyan) (left panel). Structural alignment of human GSDME NT (brown) with human PJVK NT (Cyan) both generated from the mouse GSDMA3 NT template-based model (middle panel). Structural alignment of mouse GSDMA3 NT (6CB8) (brown) with the template-based model generated for jawless Chordate lancelet fish ( Branchiostoma floridae ) <t>GSDM</t> NT (Cyan) (right panel). B Measurement of LDH release, IL-1β release, and Yo-Pro-1 + uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express human full length PJVK, PJVK NT , full length GSDMD, or GSDMD NT . C Measurement of LDH release, IL-1β release, and Yo-Pro-1 + uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express lancelet GSDM NT (blue), shark PJVK NT , or GSDME NT (dark yellow) or mouse GSDMD NT (red). In B and C, each point represents an individual assay with at least n =3 to 6 different assays. * p <0.05, ** p <0.01, and *** p <0.001; Kruskal-Wallis test with Dunn’s multiple comparison post-test
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    Full-length or N-terminal pejvakin expression does not induce pyroptosis. A Structural alignment of the molecular models of mouse GSDMA3 NT (6CB8) (brown) with the model for the template generated human PJVK NT (cyan) (left panel). Structural alignment of human GSDME NT (brown) with human PJVK NT (Cyan) both generated from the mouse GSDMA3 NT template-based model (middle panel). Structural alignment of mouse GSDMA3 NT (6CB8) (brown) with the template-based model generated for jawless Chordate lancelet fish ( Branchiostoma floridae ) GSDM NT (Cyan) (right panel). B Measurement of LDH release, IL-1β release, and Yo-Pro-1 + uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express human full length PJVK, PJVK NT , full length GSDMD, or GSDMD NT . C Measurement of LDH release, IL-1β release, and Yo-Pro-1 + uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express lancelet GSDM NT (blue), shark PJVK NT , or GSDME NT (dark yellow) or mouse GSDMD NT (red). In B and C, each point represents an individual assay with at least n =3 to 6 different assays. * p <0.05, ** p <0.01, and *** p <0.001; Kruskal-Wallis test with Dunn’s multiple comparison post-test

    Journal: BMC Biology

    Article Title: Evolutionary analyses of the gasdermin family suggest conserved roles in infection response despite loss of pore-forming functionality

    doi: 10.1186/s12915-021-01220-z

    Figure Lengend Snippet: Full-length or N-terminal pejvakin expression does not induce pyroptosis. A Structural alignment of the molecular models of mouse GSDMA3 NT (6CB8) (brown) with the model for the template generated human PJVK NT (cyan) (left panel). Structural alignment of human GSDME NT (brown) with human PJVK NT (Cyan) both generated from the mouse GSDMA3 NT template-based model (middle panel). Structural alignment of mouse GSDMA3 NT (6CB8) (brown) with the template-based model generated for jawless Chordate lancelet fish ( Branchiostoma floridae ) GSDM NT (Cyan) (right panel). B Measurement of LDH release, IL-1β release, and Yo-Pro-1 + uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express human full length PJVK, PJVK NT , full length GSDMD, or GSDMD NT . C Measurement of LDH release, IL-1β release, and Yo-Pro-1 + uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express lancelet GSDM NT (blue), shark PJVK NT , or GSDME NT (dark yellow) or mouse GSDMD NT (red). In B and C, each point represents an individual assay with at least n =3 to 6 different assays. * p <0.05, ** p <0.01, and *** p <0.001; Kruskal-Wallis test with Dunn’s multiple comparison post-test

    Article Snippet: The different GSDMs for human PJVK FL (QOZLH3) and PJVK NT (QOZLH3, amino acids 1–237), mouse GSDMD NT (QQ9D8T2, amino acids 1–276), shark GSDME NT (K4FYP8, amino acids 1–266), shark PJVK NT (A0A4W3IS58, amino acids 1–236), and lancelet GSDM NT (C3VN8, amino acids 1–249) were ordered from GenScript and cloned into the pcDNA3.1+N-MYC vector, except for human GSDMD-FLAG and human GSDMD NT -FLAG expression vectors that were kindly provided by F. Saho (National Institute of Biological Sciences, Beijing, China).

    Techniques: Expressing, Generated, Comparison